Abstrakt

Silver nanoparticles (AgNPs) prepared and stabilized by diverse biologically activesubstances seem to be especially useful in diverse biological and medical applications.The combination of AgNPs with bioactive substances, such as antioxidants, can leadto the development of new systems of desired anticancer properties. In this research,AgNPs were prepared with the use of diverse antioxidant combinations including gal-lic acid (GA), ()-epicatechin-3-gallate (EGCG), and caffeine (CAF). The insightfulphysicochemical characteristic revealed that each type of AgNPs exhibited sphericalshape, comparable size distribution and negative surface charge. Surface-enhancedRaman spectroscopy (SERS) delivered the information about the chemistry of AgNPstabilizing layers, which turned out to be a crucial factor tuning toxicity of AgNPstoward murine B16 melanoma cells (B16-F0) and human skin melanoma (COLO 679)cells. EGCGAgNPs were the most cytotoxic among all the investigated AgNPs. Theystrongly reduced the activity of mitochondria, damaged cell membrane integrity, andpenetrated inside the cells causing DNA damage. In turn, the toxicity of GAAgNPsstrongly manifested via the induction of oxidative stress in the cells. It was found thatCAFGAAgNPs exhibited the lowest toxicity toward the melanoma cells, whichproved that a proper combination of antioxidants enable to prepare AgNPs of differ-entiated toxicity. It was established that human skin melanoma cells were signifi-cantly more sensitive to AgNPs than the murine melanoma cells.